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          植物多重抗逆的分子學機理

            本團隊的研究工作主要集中在細胞膜表面受體激酶的功能分析,以及ROS介導的細胞凋亡及早衰分子調控機理的研究。本團隊的研究目的是闡明細胞表面受體激酶是如何參與胞內ROS介導的信號轉導途徑,以便今后能利用基因工程改造現有農作物,使其更有效地調控ROS的積累,從而抵抗多種逆境。

            本團隊已經將在前期工作中克隆的所有170個LRR-RLKs通過體外DNA重組技術轉移到表達載體中,并在細胞凋亡弱雙重突變體bak1-3 bkk1-1中進行了超表達,已經篩選到一個抑制bak1-3 bkk1-1細胞凋亡表型的轉基因植物,克隆了其中的超表達轉基因。bak1-3 bkk1-1是本團隊篩選到的一個弱雙突變體,與無效雙突變體不同,該突變體不會在苗期完全致死,表現為葉片提前衰老,可以用來大規模的篩選細胞凋亡抑制子。目前已經篩選了1.6萬個轉基因植株,從中鑒定出16個突變體,其細胞凋亡的表型被不同程度地抑制。已經通過TAIL-PCR等分子生物學手段確定了這些突變體中的T-DNA插入位點,目前正在對它們所調控的分子機理做深入研究。

          團隊負責人簡介

            黎家,男,漢族,1963年4月生,江蘇太倉人,博士、教授、博士生導師,現任蘭州大學生命科學學院院長,2003年獲得俄克拉荷馬大學Junior Faculty Award,2008年被教育部聘為“長江學者獎勵計劃”特聘教授,2009年入選“甘肅省領軍人才”,2012年入選中組部第八批“千人計劃(創新人才長期項目)”專家。

            黎家教授1980年-1984年在蘭州大學生物系學習并獲得學士學位。1984年-1986年在湖南師范大學生物系任助教。1986年—1989年在中科院植物研究所學習并獲得碩士學位。1990年-1991年在中科院植物研究所任助研。1991年-1995年在美國弗吉尼亞理工大學獲植物生理學博士學位。1997年-2002年在美國密蘇里大學哥倫比亞分校從事博士后研究。2002年-2010年在美國俄克拉荷馬大學植物與微生物系任助理教授及副教授(終身教職)。通過全球競聘獲任蘭州大學生命科學學院院長,于2009年3月全職回國工作。2009年作為團隊帶頭人入選教育部“長江學者和創新團隊發展計劃”。

            黎家教授主要社會兼職有:中國植物生理學會常務理事;中國細胞生物學學會理事;甘肅省植物生理學會理事長;《Food and Energy Security》副主編(Wiley-Blackwell 出版社);《遺傳》副主編;《Journal of Integrative Plant Biology》、《Journal of Genetics and Genomics》、《Frontiers In Biology》、《Plant Signaling and Behavior》編委;20多種國際主要學術刊物審稿人;國家基金委細胞生物學二審專家。

            黎家教授長期從事植物受體激酶及植物激素油菜素內酯領域的研究,是相關研究領域的國際知名學者。他及他領導的團隊分離到多個關鍵基因,對闡明油菜素內酯的合成、代謝調控及信號轉導的分子機理做出了許多開創性的貢獻。相關工作在國際重要學術期刊——《Cell》、《Developmental Cell》、《Current Biology》、《Proc. Natl. Acad. Sci. USA》、《Plant Cell》、《Plant Journal》、《Current Opinion in Plant Biology》、《PLoS Genetics》——等上發表。其中黎家教授以第一作者發表于《Cell》的關于發現油菜素內酯共受體激酶BAK1的原創性研究已經成為該研究領域的經典之作,年平均被引用數及被引用總數在所有有關油菜素內酯領域的SCI論文中均名列前茅。

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